AUTOFLUORESCENCE OF TEAR PROTEINS
Keywords:
tears, tear proteins, autofluorescence
Abstract
Tears contain many different proteins which can act as endogenous fluorescence biomarkers. This biological fluid is not used in clinical practice yet. The aim of this work is an experimental analysis of final fluorescence intensity of tear´s proteins from right and left eye of healthy subjects by synchronous fluorescence fingerprint. Tears of the healthy subjects exhibited the final protein fluorescence as the fluorescence peak placed at λ = 280 nm/ ∆λ 70 nm. The fluorescence analysis might be a non-traditional methodology for an early rapid diagnosis of eye diseases in next experimental analysis and in clinical practice.
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References
1. Gillan, W.D.H.: Tearbiochemistry: a review. S Afr Optom, 69, 2010, 100-106.
2. Dartt, D.A.: Tear Lipocalin: Structure and Function. Ocul Surf, 9, 2011, 126- 138.
3. McDermott, A.M.: The Role of Antimicrobial Peptides at the Ocular Surface. Ophthalmic Res, 41, 2009, 60–75.
4. Sack, R.A., Conradi, L., Krumholz, D. et al.: Membrane array characterization of 80 chemokines, cytokines and growth factors in open- and closed-eye tears:
angiogenin and other defense system constituents. Inv Ophthal Vis Sci, 6, 2005, 1228-1238.
5. Dreyfuss, J.L., Regatieri, C.V., Coelho, B. et al.: Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis. An Acad Bras Cienc, 87,
2015, 455-62.
6. González, N.: Evaluation of inter-day and inter-individual variability of tear peptide/protein profiles by MALDI-TOF MS analyses. Mol Vis, 18, 2012, 1572–1582.
7. Markoulli, M., Papas, E., Petznick, A. et al.: Validation of the flush method as an alternative to basal or reflex tear collection. Curr Eye Res, 36, 2011, 198-207.
8. Ng, V., Cho, P., Mak, S. et al.: Variability of tear protein levels in normal young adults: between-day variation. Graefes Arch Clin Exp Ophthalmol, 238,2000,
892-899.
9. Green-Church, K.B., Nichols, K.K., Kleinholz, N.M. et al.: Investigation of the human tear film proteome using multiple proteomic approaches. Mol Vis, 14, 2008, 456-70.
10. Tiffany, J.M.: The normal tear film. Dev Ophthal mol, 41, 2008, 1-20.
11. Guľašová, Z., Tomečková, V., Bilecová-Rabajdová, M. et al.: Monitoring of Thoracic Aortic Aneurysm in Blood by Fluorescence Spectroscopy. Am J Med Biol Res, 3, 2015, 128-132.
12. Berezin, M.Y., Achilefu, S.: Fluorescence life time measurements and biological imaging. Chem Rev, 110, 2010, 2641-2684.
13. Tomečková, V., Bohó, A., Komanický V., Tomečko, M., Guľašová, Z.: Monitorovanie rôznych srdcových ochorení z autofluorescencie krvi. Ateroskleróza, 19, 2015, 694.
14. Zhang X. Fales A.,Vo-Dinh, T.: Time Resolved Synchronous Fluorescence for Biomedical Diagnosis. Sensors, 15, 2015, 21746–21759.
15. Bohó, A., Tomečková, V., Sedláková, E.: Využitie fluorescenčnej analýzy pri včasnej diagnostike akútneho koronárneho syndrómu. CardiologyLetters, 21, 2012,
124-130.
16. Zhu, W., Zhao, Z., Guo, X.,Hong, X.: Study on serum fluorescence spectra based on wavelet transform. Afr J Biotech, 9, 2010, 892-899.
2. Dartt, D.A.: Tear Lipocalin: Structure and Function. Ocul Surf, 9, 2011, 126- 138.
3. McDermott, A.M.: The Role of Antimicrobial Peptides at the Ocular Surface. Ophthalmic Res, 41, 2009, 60–75.
4. Sack, R.A., Conradi, L., Krumholz, D. et al.: Membrane array characterization of 80 chemokines, cytokines and growth factors in open- and closed-eye tears:
angiogenin and other defense system constituents. Inv Ophthal Vis Sci, 6, 2005, 1228-1238.
5. Dreyfuss, J.L., Regatieri, C.V., Coelho, B. et al.: Altered hyaluronic acid content in tear fluid of patients with adenoviral conjunctivitis. An Acad Bras Cienc, 87,
2015, 455-62.
6. González, N.: Evaluation of inter-day and inter-individual variability of tear peptide/protein profiles by MALDI-TOF MS analyses. Mol Vis, 18, 2012, 1572–1582.
7. Markoulli, M., Papas, E., Petznick, A. et al.: Validation of the flush method as an alternative to basal or reflex tear collection. Curr Eye Res, 36, 2011, 198-207.
8. Ng, V., Cho, P., Mak, S. et al.: Variability of tear protein levels in normal young adults: between-day variation. Graefes Arch Clin Exp Ophthalmol, 238,2000,
892-899.
9. Green-Church, K.B., Nichols, K.K., Kleinholz, N.M. et al.: Investigation of the human tear film proteome using multiple proteomic approaches. Mol Vis, 14, 2008, 456-70.
10. Tiffany, J.M.: The normal tear film. Dev Ophthal mol, 41, 2008, 1-20.
11. Guľašová, Z., Tomečková, V., Bilecová-Rabajdová, M. et al.: Monitoring of Thoracic Aortic Aneurysm in Blood by Fluorescence Spectroscopy. Am J Med Biol Res, 3, 2015, 128-132.
12. Berezin, M.Y., Achilefu, S.: Fluorescence life time measurements and biological imaging. Chem Rev, 110, 2010, 2641-2684.
13. Tomečková, V., Bohó, A., Komanický V., Tomečko, M., Guľašová, Z.: Monitorovanie rôznych srdcových ochorení z autofluorescencie krvi. Ateroskleróza, 19, 2015, 694.
14. Zhang X. Fales A.,Vo-Dinh, T.: Time Resolved Synchronous Fluorescence for Biomedical Diagnosis. Sensors, 15, 2015, 21746–21759.
15. Bohó, A., Tomečková, V., Sedláková, E.: Využitie fluorescenčnej analýzy pri včasnej diagnostike akútneho koronárneho syndrómu. CardiologyLetters, 21, 2012,
124-130.
16. Zhu, W., Zhao, Z., Guo, X.,Hong, X.: Study on serum fluorescence spectra based on wavelet transform. Afr J Biotech, 9, 2010, 892-899.
How to Cite
1.
Gabriela Glinská, Vladimíra Tomečková, Kristína Krajčíková. AUTOFLUORESCENCE OF TEAR PROTEINS. Med. res. chronicles [Internet]. 2016Oct.31 [cited 2024May8];3(05):442-9. Available from: https://medrech.com/index.php/medrech/article/view/197
Section
Original Research Article
