ASSESSMENT OF HUMAN PAPILLOMA VIRUS SUBTYPES BY POLYMERASE CHAIN REACTION AND THEIR IMPACT ON THE DEGREE OF DYSPLASIA IN ORAL LEUKOPLAKIA
Abstract
Aim: The study was intended to determine the prevalence of HPV subtypes in patients with oral leukoplakia & assess the possible correlation between HPV and dysplastic changes in oral leukoplakia.
Materials and Methods: Research was carried out in 25 patients with oral leukoplakia. HPV DNA was determined in incisional biopsy specimens by PCR and HPV subtypes by direct DNA sequencing.
Results: Statistical analysis was done using the SPSS version 20.0. HPV DNA was detected in 15 patients (60%) out of 25 leukoplakia patients. HPV 16 and 18 were detected in 8 patients (32%), HPV 18 was detected in 6 patients (24%) and HPV16 alone was detected in 1patient (4%). 10 patients (40%) were negative for HPV DNA. There was a statistical significant correlation between HPV DNA and degree of dysplasia (p value 0.037).
Conclusion: An increased prevalence of HPV infection was found in oral leukoplakia; It was observed that HPV might play a crucial role in malignant transformation of potentially malignant lesion like oral leukoplakia.
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References
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