THE CORRELATION BETWEEN SALIVARY AND SERUM CORTISOL LEVELS IN THE HEALTHY POPULATION

  • Blertina Dyrmishi Endocrinologist, department of internal medicine, Hygeia Hospital, Tirana, Albania
  • Taulant Olldashi Surgen, Hygeia Hospital, Tirana, Albania
  • Entela Puca Endocrinologist, Immunologist, Head of Endocrine Department, UHC Mother Teresa, Tirana, Albania
  • Prof asc Zamira Ylli Endocrinologist, Immunologist, Head of Endocrine Department, UHC Mother Teresa, Tirana, Albania
  • Elvana Rista Nephrologist, department of internal medicine, Hygeia Hospital, Tirana, Albania
  • Prof Agron Ylli Endocrinologist, Immunologist, Head of Endocrine Department, UHC Mother Teresa, Tirana, Albania
Keywords: salivary cortisol, serum cortisol, correlation

Abstract

Aim: The aim of the study was to evaluate the correlation between salivary and serum cortisol levels in the healthy population.

Methods: 30 healthy women and men, aged from 22 to 53 years old, were included in our study. All the participants were healthy, without any known disease. Two samples of salivary and serum cortisol were collected from all the participants in the study, in the morning and in the afternoon, before and after work. The mean age was 33 ± 14 SD years old. 60% (18) were females and 40% (12) were males. All the participants were within normal weight range, the mean BMI was 23.5 kg/m². All the subjects followed the rules of salivary sample collection.

Results: The mean age of the healthy subjects was 33 ± 14 years old. The mean baseline morning cortisol level of the study population was 159.6 ng/ml (SD: 41.7; normal range 55-230 ng/ml, 8:00 AM) and the mean salivary cortisol level in the morning was 3.2 µg/dL (SD: 2.4µg/dL; normal range 1-11.3 µg/dL). After work, at 4:00 PM, the mean baseline serum cortisol was 100.1 ng/ml (SD: 30.7; normal range 28-140 ng/ml) and the mean salivary cortisol level after work was 1.21 µg/dl (SD: 0.59; normal range 0.2-2.7 µg/dl). Baseline salivary cortisol correlated significantly with serum salivary cortisol before and after work; at 8:00 AM and 4:00
PM; p <0.05 in the morning and p< 0.0001 after work. The Pearson’s coefficients were 0.44 in the morning and 0.7 in the afternoon.

Conclusions: In our study we found a correlation between salivary and serum cortisol levels in both samples, in the morning and in the afternoon. The salivary cortisol levels correlated significantly with the serum cortisol levels (r = 0.44, p< 0.05 in the morning and r = 0.7, p<0.00.1 in the afternoon).

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References

1. Ballarg PL, Carter JP, Graham BS, Baxter JD. A radioreceptor assay for evaluation of the plasma glucocorticoid activity of natyral and sintetic steroids in man. J Clin Endorinol Metab 1975;41(2): 290-304.
2. Amir H. Hamrahian, MD; Tawakalitu S, et al. Measurements of serum Free Cortisol In critically ill Patient. N Engl J Med. 2004; 350 (4): 1629-1638.
3. Huang W, Kalhorn TF, Baillie M, et al. Determination of free and total cortisol in plasma and urine by liquid Chromatography-tandem mass spectrometry. Ther Drug Moint 2007;29(2) :215-224.
4. Walker RF, Riad-Famhy D, Read GF. Adrenal status assessed by direct radioimmunoassay of cortisol in whole saliva or parotid saliva. Clin Chem 1978; 24 (9): 1460-1463.
5. Vining RF; McGinley RA; Symons RG. Hormones in saliva: mode of entry and consequent implications for clinical interpretatio. Clin Chem. 1983; 29 (10):1752-1756.
6. Finding JW & Ralf H. Screening and diagnosis of Cushing’s syndrome. Endocrinology and Metabolism Clinic of North America. 2005, 34; 385-402
7. Yaneva M, Mosnier-Pudar H, Dugue MA, Grabar S, Fulla Y & Bertagna X. Midnight salivary cortisol for the intial diagnosis of Cushing’s syndrome of various causes. Journal of Clinical Endocrinology and Metabolism 2004, 89( 7): 3345-3351.
8. Castro M, Elias PC, Martinelli CE, Jr, Antonini SR, Santiago L & Moreira AC. Salivary cortisol as a tool for physiological studies and diagnostic strategies. Brazilian Journal of Medical and Biological Research 2000; 33, 1171-1175.
9. Chen YM, Cintron NM, Whitson Pa. Long-term storage of salivary cortisol samples at room temperature. Clin Chem 1992; 38 (2):304.
10. Raff H, Homar PJ, Burn Ea. Comparison of two methods for measuring salivary cortisol. Clin Chem 2002: 48:207-208.
11- Mosnier-Pudar, H, Thomopoulos P, Bertagna X, et al. Long Distance and long –term follow–up of a patient with intermittent Cushing disease by salivary cortisol measurements. Eur J Endocrinol 1995; 133:313-316.
12. Papanicolaou Da, Mullen N, Kyrou I, Nieman LK. Nightime salivary cortisol: a usefull test for the diagnosis of Cushing’s syndrome. J Clin Endocrinol Metab 2002; 87: 4515-4521.
13. R.K. Schindelm and al. salivary cortisol as an alternative for serum cortisol in the low-dose adrenocorticotropic hormone stimulation test. J Endocrinol. Invest
2010; 33: 92-95.
14. VanBruggen MD and al. The relationship between serum and salivary cprtisol levels in response to different intensities of exercise. Int J Sports Physiol Perform, 2011; 6 (3) : 396-407
How to Cite
1.
Blertina Dyrmishi, Taulant Olldashi, Entela Puca, Prof asc Zamira Ylli, Elvana Rista, Prof Agron Ylli. THE CORRELATION BETWEEN SALIVARY AND SERUM CORTISOL LEVELS IN THE HEALTHY POPULATION. Med. res. chronicles [Internet]. 1 [cited 2024May4];3(1):91-6. Available from: https://medrech.com/index.php/medrech/article/view/148
Issue
Vol. 3 No. 1 (2016): Jan-Feb
Section
Original Research Article

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