CHANGES OF BLOOD PLASMA PROTEINS DURING SELECTED CARDIOVASCULAR DISEASES

  • Zuzana Guľašová Dept. of Experimental Medicine and Dept. of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Slovak Republic,
  • Vladimíra Tomečková Dept. of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Slovak Republic
  • Vladimír Komanický Institute of Physics, Faculty of Science, Pavol Jozef Šafárik University in Košice, Slovak Republic
  • Miroslava Rabajdová Dept. of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Slovak Republic
  • František Sabol Dept. of Cardiovascular Surgery, Faculty of Medicine, Pavol Jozef Šafárik University in Košice and VUSCH, Košice, Slovak Republic
  • Mária Mareková Dept. of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Slovak Republic
Keywords: Blood serum, cardiovascular diseases, Fluorescence spectroscopy, Atomic force microscopy

Abstract

Cardiovascular diseases are one of the most dangerous diseases in the world and that is why it is necessary constantly research and try to study these diseases not only with traditional methods but also with new techniques. This study describes laboratory methods that are applicable for the rapid early screening of cardiovascular diseases. Blood plasma of patients with thoracic aortic aneurysm, aortic valve stenosis and aortic valve regurgitation was subjected to experimental biochemical analysis by fluorescence analysis and atomic force microscopy. The structural and metabolic changes have been studied in blood plasma of patients with a thoracic aneurysm in comparison with the samples of healthy individuals. The results of fluorescence analysis showed a significant decrease of autofluorescence in blood plasma of patients in comparison with healthy subjects. Atomic force microscopy confirmed structural changes in the blood of patients in comparison with the blood of healthy subjects. The results of using new experimental laboratory methods in future could contribute to the improvement of diagnosis of selected cardiovascular diseases.

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References

1. Zomer E., Leach R., Trimmer C., Lobstein T., Morris S., James W.P., Finer N. Effectiveness and costeffectiveness of interventions that cause weight loss and reduce
the risk of cardiovascular disease. Diabetes Obes Metab. 2016, doi: 10.1111/dom.12792.
2. Goumans M.J., Liu Z., ten Dijke P. TGF-β signaling in vascular biology and dysfunction. Cell Res. 19, 2009, 116- 127.
3. Fanjul-Fernández M., Folgueras A.R., Cabrera S., López-Otín C. Matrix metalloproteinases: evolution, gene regulation and functional analysis in mouse models. Biochim Biophys Acta. 1803(1), 2010, 3-19.
4. Stoner L., Lucero A.A., Palmer B.R., Jones L.M., Young J.M., Faulkner J. Inflammatory biomarkers for predicting cardiovascular disease. Clin Biochem. 46(15), 2013, 1353-1371.
5. Tan M., Liang W., Luo X., Gu Y. Fluorescence Spectroscopy Study on the Interaction between Evodiamine and Bovine Serum Albumin. J Chem. 2013, 2013, 1-6.
6. Palta S., Sara R., Palta A. Overview of the coagulation system. Indian J Anaesth. 58(5), 2014, 515-523.
7. Young Ch., Jeongseon K. Effect of probiotics on blood lipid concentrations: A meta-analysis of randomized controlled trials. Medicine. 2015, 94(43),
e1714.
8. Tehrani D.M., Zhao Y., Blaha M.J., Mora S., Mackey, R.H., Michos E.D., Budoff M.J., Cromwell W., Otvos J.D., Rosenblit P.D., Wong N.D. Discordance of low-density lipoprotein and highdensity lipoprotein cholesterol particle versus cholesterol concentration for the prediction of cardiovascular disease in patients with metabolic syndrome and diabetes mellitus (from the multi-ethnic study of atherosclerosis [MESA]). Am J Cardiol. 2016, 9149(16), 30427-30431.
9. Äijälä M., Malo E., Santaniemi M., Bloigu R., Silaste M.L., Kesäniemi Y.A., Ukkola O. Dietary sodium intake and prediction of cardiovascular events. Eur J Clin Nutr. 69(9), 2015, 1042-1047.
10. Haarhaus M., Arnqvist H.J., Magnusson P. Calcifying human aortic smooth muscle cells express different bone alkaline phosphatase isoforms, including the novel B1x isoform. J Vasc Res. 50(2), 2013, 167-174.
11. Mcgovern A.P., Delusignan S., Vanvlymen J., Liyanage H., Tomson C.R., Gallagher H., Rafiq M., Jones S. Serum phosphate as a risk factor for cardiovascular events in people with and without chronic kidney disease: a large community-based cohort study. PLoS One. 8(9), 2013, e74996.
12. Von Haehling S., Jankowska E.A., van Veldhuisen D.J., Ponikowski P., Anker S.D.Iron deficiency and cardiovascular disease. Nat Rev Cardiol. 12, 2015, 659-669.
13. Chiuve S.E., Sun Q., Curhan G.C., Taylor E.N., Spiegelman D., Willett W.C., Manson J.E., Rexrode K.M., Albert C.M. Dietary and plasma magnesium and risk of coronary heart disease among women. J Am Heart Assoc. 2(2), 2013, e000114.
14. Cary T., Pearce J. Aortic stenosis: pathophysiology, diagnosis, and medical management of nonsurgical patients. Crit Care Nurse. 33(2), 2013, 58-72.
15. Delgado V., Biermasz N.R., Van Thiel S.Z., Ewe S.H., Marsan N.A., Holman E.R., Feelders R.A., Smit J.W., Bax J.J., Pereira A.M. Changes in heart valve structure and function in patients treated with dopamine agonists for prolactinomas, a 2-year follow-up study. Clin Endocrinol Oxford. 77(1), 2012, 99-105.
16. Lee Ch.K., Wang Y.S., Huang L.S., Lin S. Atomic force microscopy: Determination of unbinding force, off rate and energy barrier for protein-ligand
interaction.Micron. 38, 2007, 446-461.
17. Carvalho F.A., De Oliveira S., Freitas T., Goncalves S., Santos N.C. Variations on fibrinogen-erythrocyte interactions during cell aging. PLoS One. 2011, 6(3), e18167.
18. Guedes A.F., Carvalho F.A., Malho I., Lousada N., Sargento L., Santos N.C. Atomic force microscopy as a tool to evaluate the risk of cardiovascular diseases in patients. Nat Technol. 2016,doi:10.1038/nnano.2016.52.
19. Lu P., Takai K., Weaver V.M., Werb Z. Extracellular matrix degradation and remodeling in development and disease. Cold Spring Harb PerspectBiol. 3(12),2011, a005058.
How to Cite
1.
Zuzana Guľašová, Vladimíra Tomečková, Vladimír Komanický, Miroslava Rabajdová, František Sabol, Mária Mareková. CHANGES OF BLOOD PLASMA PROTEINS DURING SELECTED CARDIOVASCULAR DISEASES. Med. res. chronicles [Internet]. 1 [cited 2024May8];3(05):450-8. Available from: https://medrech.com/index.php/medrech/article/view/198
Issue
Vol. 3 No. 05 (2016): Sep-Oct
Section
Original Research Article