CLINICAL SIGNIFICANCE OF T LYMPHOCYTE SUBSETS AND HLA-DR IN PERIPHERAL BLOOD OF PATIENTS WITH SEVERE PREECLAMPSIA
Objective: To observe the variation of T lymphocyte subsets and human histocompatibility antigen (HLA-DR) in peripheral blood of patients with severe preeclampsia（ SPE).
Method: Peripheral blood from 30 patients with SPE (SPE group), 30 healthy pregnant women (healthy pregnant group) and 18 healthy un-pregnant women (healthy un-pregnant group) was a collected． The percentage of CD4+T, CD8+T and HLA-DR in each group were compared by Flow cytometry (FCM).
Results: There was no difference in the expression of CD4+T in each group ((35.6±5.4)%VS (37.8±8.9), (35.6±5.4)%VS(37.1±9.6)%). The expression of CD8+T in SPE group was significantly higher than those in the healthy pregnant group ((32.1±7.4)% VS(26.9±7.7)%, P＜ 0.01) and in the healthy un-pregnant group ((32.1±7.4)% VS(25.4±7.6)%, P＜ 0.01). The expression of HLA-DR in SPE group was significantly lower than that in healthy pregnant group ((43.7±12.7)% VS(58.3±13.9)%； P＜ 0.01)．
Conclusions: Higher expression of CD8+T was found in peripheral blood of SPE patients which lead to T lymphocyte subsets imbalance. The decrease of HLA-DR may contribute to the pathologic process of SPE．
 Joe Chong, Yang Xiaomei, Lin Qi de. Advance in the epidemiology of preeclampsia. China family planning and obstetrics and gynecology, 2013, (06): 5-
 Ding Hong, Zhang Jianping, et al. Weeks of mechanics, the change trend of the thirty years of epilepsy. Chinese Journal of perinatal medicine, 2003, (01): 10-12.
 Lu L, Qu Y, Tang J, et al. Risk factors associated with late preterm births in the underdeveloped region of China: A cohort study and systematic review.
Taiwan J Obstet Gynecol, 2015,54(6):647-653.
 Lamarca B. The role of immune activation in contributing to vascular dysfunction and the pathophysiology of hypertension during preeclampsia. Minerva Ginecol, 2010,62(2):105-200.
 Luo Xin, Hong Bo. Advance in molecular biology of the pathogenesis of preeclampsia. Chinese Journal of practical gynecology and obstetrics, 2012, (04): 309-311.
 Lin Li, Xiong Qing, Li Youping, et al. A systematic review of the study of human leukocyte antigen-D in patients with preeclampsia/eclampsia. Chinese journal
of evidence-based medicine, 2006, (02): 117-123.
 Cornelius DC, Amaral LM, Harmon A, et al. An increased population of regulatory T cells improves the pathophysiology of placental ischemia in a rat model of preeclampsia. Am J Physiol Regul Integr Comp Physiol,2015,309(8): R884-891.
 ACOG practice bulletin. Diagnosis and management of preeclampsia and eclampsia. Number 33, January 2002. Obstet Gynecol, 2002,99(1):159-167.
 Li Yingfeng, Zhou Huahui. Comprehensive change and significance of pregnant women with serum Creactive protein and cystatin C levels in pregnancy induced hypertension. Modern journal of integrated traditional Chinese and western medicine, 2011, (02): 156-157.
 Liu Huilin, Liu Guihua, Tian Zhaoxing. Changes of lymphocytes in blood in patients with sepsis. The critical care medicine, 2014,26 (3): 148-152.
 Zhang Jianping, Chen Libin, Liu Yinglin, et al. Observation of T lymphocyte subsets and NK cells in peripheral blood in the middle and late pregnancy. Progress in obstetrics and gynecology, 2002, (02): 125-127.
 Wang Qiuwei, Xu Peizhen, Yu Bin, et al. Clinical significance of detection of T lymphocyte subsets in patients with pregnancy-induced hypertension. Journal of clinical medicine in practice, 2003, (04): 367-369.
 Sun Li, Liu Chunxia, Cheng Jianzhen, et al. Measurement of T-lymphocyte subsets in peripheral blood of patients with pregnancy induced hypertension.
Journal of Zhangjiakou Medical College, 2000, (01): 13-14.
 Zhou Dongxia. Clinical significance of serum leptin levels in patients with pregnancy-induced hypertension and detection of B and T cell subsets in peripheral blood. Journal of radioimmunology, 2007, (06): 519-520.
 Darmochwal-Kolarz D, Saito S, Rolinski J, et al. Activated T lymphocytes in pre-eclampsia. Am J Reprod Immunol, 2007,58(1):39-45.
 Qin Caijuan. Correlation study between D- two polymers, T lymphocyte subsets and C-reactive protein in hypertensive disorder complicating pregnancy. China
medical herald, 2012, (17): 22-25.
 Zhong Xiaohong. Study on the dynamic changes of plasma D- two, T lymphocyte subsets and C- reactive protein in pregnant women with pregnancy-induced hypertension. Practical preventive medicine, 2015, (07): 866-868.
 Biggar RJ, Poulsen G, Ng J, et al. HLA antigen sharing between mother and fetus as a risk factor for eclampsia and preeclampsia. Hum Immunol,
 Ogge G, Romero R, Chaiworapongsa T, et al. Leukocytes of pregnant women with small-for-gestational-age neonates have a different phenotypic and
metabolic activity from those of women with preeclampsia. J Matern Fetal Neonatal Med, 2010,23(6):476-487.
 Lv Lianzheng, Lin Xin, money Lei, et al. Expression and clinical significance of HLA-DR in peripheral blood mononuclear cells in patients with preeclampsia. Journal of Jiangsu University (medical edition), 2015, (06): 482-490.