• Oluwayemisi A. Olorode Department of Pharmaceutical Microbiology and Biotechnology, Niger Delta University, Wilberforce Island, Bayelsa, Nigeria
  • Emmanuel A. Bamigbola Depart of Pharmaceutics and Pharmaceutical Technology Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa, Nigeria
  • Dokubo D. Dorari Depart of Pharmaceutics and Pharmaceutical Technology Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa, Nigeria
Keywords: Automated Teller Machine (ATM), Formulated Novel disinfectant, Potency evaluation, Bacteria


An increasing number of people has used the Automated Teller Machine (ATMs) by the year, but less was known about their bacteria colonization status. Do these machines harbor pathogenic bacteria? This work was carried out between March and November 2015 to isolate the pathogenic bacteria associated with ATMs and to evaluate the potency of the formulated novel (Darol) and commonly sold disinfectants (Izal, Domitol) on these bacteria isolates. Samples were collected from thirty-two (32) different Automated Teller Machines located in different banks, within Yenagoa metropolis, with wet sterile swabs and cultured, isolated and identified in the laboratory using selective media and microbiological standard procedures respectively. The bacteria suspension of pure isolates were standardized with 0.5 Mc Farland Turbidity Standard and subjected to antimicrobial susceptibility testing of the disinfectants at different concentrations (2% 4% 6% 8% 10% and 12%) using cup plate method. Seventy-nine (79) bacteria strains which include Staphylococcus aureus, 37(46%); Klebsiella sp., 19 (24%); Pseudomonas 6 (7.5%) and Bacillus 6 (7.5%) were isolated and identified. Statistical analysis showed no significant difference in the potency of the test disinfectants (p<0.05) at different concentrations except at 2% between Darol and others for Klebsiella and Pseudomonas species. In conclusion, findings have shown that the potency of the test disinfectants increases with increase in concentrations and Darol mean value 75.5 (43.1%) was most potent, followed by Damitol mean value 54.1 (31%) and the least was Izal mean value 45.4 (26%).


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How to Cite
Oluwayemisi A. Olorode, Emmanuel A. Bamigbola, Dokubo D. Dorari. POTENCY EVALUATION OF A FORMULATED NOVEL DISINFECTANT ON PATHOGENIC BACTERIA ISOLATED FROM AUTOMATED TELLER MACHINES (ATMs) IN BAYELSA STATE, NIGER DELTA, NIGERIA. Med. res. chronicles [Internet]. 1 [cited 2021Oct.21];4(01):123-31. Available from: https://medrech.com/index.php/medrech/article/view/223
Original Research Article