ROLE OF DIFFERENT IRRIGANTS AND INTRACANAL MEDICAMENTS AGAINST E.FECALIS: AN IN VITRO STUDY
Keywords:
Calcium Hydroxide, Chlorhexidine, E. Faecalis
Abstract
The aim of the present article was to evaluate the investigate the antimicrobial activity of 2% Chlorhexidine, 1% NaOCl , Ca(OH)2 and sterile saline against Enterococcus Faecalis infected dentin after a 7 day interval. In this study 80 premolars extracted for orthodontic purpose were used as specimen. The tooth were standardized to a length of 17 mm, the apices sealed with cyanoacrtylate and autoclaved. After with the specimen were autoclaved. They were then transferred to a broth containing E. Faecalis and incubated for 7 days. The chemomechanical preparation of the tooth was done using Race files and the specimens were irrigated with their respective irrigants. The orifices were sealed with cynocarylate and incubated for another 7 days. The coronal end was re accessed and dentinal shaving was collected using a size 5 Gates Glidden drill. The dentinal shaving were cultured and statically analyzed. Results should that though none of the medicaments were able to completely eradicate the bacteria, 2% CHX had the best results, followed by 1% NaOCl followed by Ca(OH)2. . Sterile saline was used as a control.
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References
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10 Molander A, Reit C, Dahle´n G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int J Endod 1998; 31:1-7.
11 Sundqvist G, Figdor D, Persson S, Sjo¨gren U. Microbiologic analysis of teeth with failed endodontic treatment and outcome of conservative retreatment. Oral Surg Oral Med Oral Path Oral Radiol Endod 1998;85:86-93.
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13 Tang G, Samaranayake LP, Yip HK. Molecular evaluation of residual endodontic microorganisms after instrumentation, irrigation and medication with either calcium hydroxide or Septomixine. Oral Dis 2004;10:389 –97.
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17 Safavi KE, Nichols FC. Effect of calcium hydroxide on bacterial lipopolysaccharide. J Endod 1993;19:76–8.
18 Frais S, Ng YL, Gulabivala K. Some factors affecting the concentration of available chlorine in commercial sources of sodium hypochlorite. Int Endod J
2001;34:206 –15.
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20 José F. Siqueira Jr, PhD, and Isabela N. Rôças, PhDClinical Implications and Microbiology of Bacterial Persistence after Treatment Procedures JOE —Volume 34, Number 11, November 2008
21 Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res 1987;66:1375–9.
22 Lin Y, Mickel A, Chogle S. Effectiveness of selected materials against Enterococcus faecalis: Part 3. The antibacterial effect of calcium hydroxide and hlorhexidine on Enterococcus faecalis. J Endod 2003;29:565– 6.
23 McHugh CP, Zhang P, Michalek S, Eleazer PD. pH required to kill Enterococcus faecalis in vitro. J Endod 2004;30:218 –9.
24 Tronstad L, Andreasen J, Hasselgren G, Kristerson L, Riis I. pH changes in dental tissues after root filling with calcium hydroxide. J Endod 1981;7:17– 21.
25 Gomes BPFA, Ferraz CC, Vianna ME, Berber VB, Teixeira FB, Souza-Filho FJ. In vitro antimicrobial activity of several concentrations of sodium hypochlorite
and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J 2001;34:424–8.
26 Siqueira JF, Rocas IN, Faviera A, Lima KC. Chemomechanical reduction of the bacterialpopulation in the root canal after instrumentation and irrigation with 1.0, 2.5, and 5.25% sodium hypochlorite. J Endod 2000;26:331– 4.
27 Radcliffe CE, Potouridou L, Qureshi R, et al. Antimicrobial activity of varying concentrations of sodium hypochlorite on the endodontic microorganisms
Actinomyces israelii, A naeslundii, Candida albicans, and Enterococcus faecalis. Int Endod J 2004;37:438–46.
2 Baumgartner JC, Falkler WA. Bacteria in the apical 5 mm of infected root canals. J Endod 1991;17:380 –3.3.
3 Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 1998;31:1–7.
4 Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:86 – 93.
5 Hancock HH, Sigurdsson A, Trope M, Moiseiwitsch J. Bacteria isolated after unsuccessful endodontic treatment in a North Am population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001;91:579 –86.
6 Evans M, Davies JK, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J 2002;35:221– 8.
7 Davies GE, Francis J, Martin AR, Rose L, Swain G. 1:6- di-4-chlorophenyldiguanido-hexane (_Hibitane_). Laboratory investigation of a new antibacterial agent of high potency. Br J Pharmacol 1954; 9: 192–196. 31.
8 Lawrence CA. Antimicrobial activity, in vitro, of chlorhexidine. J Am Pharmacol Assoc 1960; 49: 731–734. 32.
9 Van der Meiren L, Moriame G, Achten G, Ledoux- Corbusier M. La chlorhexidine en dermatologie. (English summary). Arch Belg Derm Syph 1958;14: 14–18.
10 Molander A, Reit C, Dahle´n G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int J Endod 1998; 31:1-7.
11 Sundqvist G, Figdor D, Persson S, Sjo¨gren U. Microbiologic analysis of teeth with failed endodontic treatment and outcome of conservative retreatment. Oral Surg Oral Med Oral Path Oral Radiol Endod 1998;85:86-93.
12 Ro¨lla G, Lo¨e H, Rindom Schio¨tt C. The affinity of chlorhexidine for hydroxyapatite and salivary mucins. J Perio Res 1970;5:90-5.
13 Tang G, Samaranayake LP, Yip HK. Molecular evaluation of residual endodontic microorganisms after instrumentation, irrigation and medication with either calcium hydroxide or Septomixine. Oral Dis 2004;10:389 –97.
14 Froeman PC, Barnes IE (1990) A review of calcium hydroxide. International Endodontic Journal 23, 283–97
15 Spangberg LSW (1994) Intracanal medication. In: Ingle JI, Bakland LK, eds. Endodontics, 4th edn. Baltimore: Williams & Wilkins, pp. 627–40.
16 Gomes BP, Ferraz CC, Vianna ME, et al. In vitro antimicrobial activity of calcium hydroxide pastes and their vehicles against selected microorganisms. Braz Dent J 2002;13:155– 61.
17 Safavi KE, Nichols FC. Effect of calcium hydroxide on bacterial lipopolysaccharide. J Endod 1993;19:76–8.
18 Frais S, Ng YL, Gulabivala K. Some factors affecting the concentration of available chlorine in commercial sources of sodium hypochlorite. Int Endod J
2001;34:206 –15.
19 Spångberg L, Engström B, Langeland K. Biologic effects of dental materials. 3. Toxicity and antimicrobial effect of endodontic antiseptics in vitro. Oral Surg Oral Med Oral Pathol 1973;36:856 –71.
20 José F. Siqueira Jr, PhD, and Isabela N. Rôças, PhDClinical Implications and Microbiology of Bacterial Persistence after Treatment Procedures JOE —Volume 34, Number 11, November 2008
21 Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res 1987;66:1375–9.
22 Lin Y, Mickel A, Chogle S. Effectiveness of selected materials against Enterococcus faecalis: Part 3. The antibacterial effect of calcium hydroxide and hlorhexidine on Enterococcus faecalis. J Endod 2003;29:565– 6.
23 McHugh CP, Zhang P, Michalek S, Eleazer PD. pH required to kill Enterococcus faecalis in vitro. J Endod 2004;30:218 –9.
24 Tronstad L, Andreasen J, Hasselgren G, Kristerson L, Riis I. pH changes in dental tissues after root filling with calcium hydroxide. J Endod 1981;7:17– 21.
25 Gomes BPFA, Ferraz CC, Vianna ME, Berber VB, Teixeira FB, Souza-Filho FJ. In vitro antimicrobial activity of several concentrations of sodium hypochlorite
and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J 2001;34:424–8.
26 Siqueira JF, Rocas IN, Faviera A, Lima KC. Chemomechanical reduction of the bacterialpopulation in the root canal after instrumentation and irrigation with 1.0, 2.5, and 5.25% sodium hypochlorite. J Endod 2000;26:331– 4.
27 Radcliffe CE, Potouridou L, Qureshi R, et al. Antimicrobial activity of varying concentrations of sodium hypochlorite on the endodontic microorganisms
Actinomyces israelii, A naeslundii, Candida albicans, and Enterococcus faecalis. Int Endod J 2004;37:438–46.
How to Cite
1.
Dr. Sujan Rijal, Dr. Sushil Subedi, Dr Arjun Bhandari. ROLE OF DIFFERENT IRRIGANTS AND INTRACANAL MEDICAMENTS AGAINST E.FECALIS: AN IN VITRO STUDY. Med. res. chronicles [Internet]. 2017Apr.30 [cited 2024Apr.29];4(02):193-7. Available from: https://medrech.com/index.php/medrech/article/view/232
Section
Original Research Article
