ESTABLISHING AN EFFECTIVE ANTIHYPERGLYCEMIC DOSE FOR SIX PLANT EXTRACTS USING THE ORAL GLUCOSE TOLERANCE TEST IN MICE WITH NORMAL PANCREATIC FUNCTION
Abstract
The oral glucose tolerance test is useful for the determination of insulin receptors sensitivity to an excess of glucose in the healthy animal. The test was carried out using mice with normal pancreatic function and the plant extracts was made from very rarely studied plant species: Tragopogon pratensis, Dorycnium pentaphyllum subsp herbaceum, Acanthus balcanicus, Tamarix ramosissima, Carduus acanthoides, taking as a reference the proven antihyperglycemic effect of tincture of Vaccinium myrtillus. After oral administration of glucose (2g/kg) in previously untreated mice, we observed an increase in plasma glucose within 30 minutes of TTGO administration, reaching a maximum of 60 minutes, with glycemic values progressively decreasing to 90 minutes and 120 minutes. The obtained average blood sugar level to 120 minutes is considerably increased compared to the initial mean before oral administration of glucose. Following testing, we established for the tinctures studied the optimum concentration at which the most powerful antihyperglycemic effect and 200 mg / kg respectively for tincture Dorycnii pentaphylli herba and 150 mg/kg for tinctures Tamaricis ramosissimae folium et flos, Tragoponis pratensis folium, Cardui acanthoiditis folium, Acanthi balcanici herba and Myrtilli fructus. After the test we concluded that all plant extracts have shown antihyperglycaemic capacity, the groups II-VII blood glucose level treated with tincture being reduced in comparison with group I
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References
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