TESTING THE HYPOGLYCEMIC AND LIPID-LOWERING EFFECT OF DORYCNIUM HERBACEUM PLANT POWDER COMPARED TO BILBERRY, USING MICE WITH STREPTOZOTOCIN DIABETES
Abstract
Diabetes mellitus is one of the most serious and most common multisystemic diseases with increasing prevalence and incidence, leading to early disability and mortality. In the pharmaceutical field, there has been an increase in the demand for natural hypoglycemic products, the management of diabetes being a challenge for the medical system. In this context, the present study aims to investigate the hypoglycemic and lipid-lowering action of plant powder derived from Dorycnium herbaceum using experimentally streptozotocininduced diabetic mice. For the induction of diabetes, streptozotocin was injected intraperitoneal in a single dose of 180 mg/kg b.w. The experiment was performed on 4 groups of 5 adult Swiss Albino Mice mice, the animals receiving daily medication at the same time for four weeks. Three control groups were used in the experiment: group I consisting of mice with normal pancreatic function, diabetic group II and diabetic group IV, treated with Vaccinium myrtillus fructus powder (50 mg / kg), a recognized product for the hypoglycemic and hypolipidemic effect . diabetic mice Group III diabetic mice were treated with Dorycnium herbaceum herba powder (50 mg / kg). At seven-day intervals, after a 12-hour diet, we determined blood glucose, cholesterol, and triglycerides, blood being harvested from the tail vein. The results obtained support the efficacy of the studied powders, normalizing the values of glycemia and lipids level in diabetes.
Downloads
References
2. Rout SP, Kar DM, Mandal PK, Hypoglycaemic activity of aerial parts of Argemone mexicana in experimental rat models, International Journal of Pharmacy
and Pharmaceutical Sciences, 3, nr. 5, 2011, 533-540.
3. A journal of the American diabetes association, 61(1A):, 2012, 1-3.
4. Frode TS, Medeirosb ZS, Animal models to test drugs with potential antidiabetic activity, Journal of Ethnopharmacology, 115, 2008, 173–183.
5. Amodu B, Itodo SE, A Novel Ethnomedicinal polyherbal formulation for the management of diabetes mellitus, Journal of Pharmacy and Biological Sciences, 5, nr. 1, 2013, 38-41.
6. Helmstädter A, Schuster N. Vaccinium Myrtillus as antidiabetic medicinal plant research through the ages, Pharmazie, 65:315–321, 2010.
7. Qin Z, Xia M, Ma J., et al, Anthocyanin supplementation improves serum LDLand HDL-cholesterol concentrations associated with the inhibition of cholesteryl ester transfer protein in dyslipidemic subjects, Am J Clin Nutrition, 90:485–492, 2009.
8. Bubueanu G, Câmpeanu G, Hypoglycaemic and cholesterol-lowering properties of an extract obtained from Vaccinium myrtillus leaves and Humulus lupulus cones Romanian Biotechnological Letters, 19, nr. 5, 2014
9. Cignarella A., Nastasi M., Cavalli E., et al., Novel lipid-lowering properties of Vaccinium Myrtillus leaves, a traditional antidiabetic treatment, in several models
of rat dyslipidemia, a comparison with ciprofibrate, Thrombosis Research, 84, nr. 5, 1996, 311–322.
10. Varut RM, Rotaru, L.T., Varut, M.C., QSPR Correlation of Physico-chemical Descriptors with the Molecular Surface Area and Rf of Ten Polyphenolic Compounds, Separated from Vegetal Extracts by TLC, REV.CHIM., 68, nr. 8, 2017, 1776- 1779.
11. Varut, RM, Rotaru, LT, GC-MS, HPLC, TLC of Dorycnium herbaceum Tincture Species and Synergistic / Antagonist Effect Testing in Combination with Antibiotics, REV.CHIM., 68, nr. 2, 2017, p. 228-231.
12. Sachin A, Shreesh KO, Vohora D. Characterisation of StreptozotocinInduced Diabetes Mellitus in Swiss Albino Mice, Global Journal of Pharmacology, 3, nr. 2, 2009, 81-84.
13. Tian HL, Li-Shun W, Zhong-Xin X., et al., Correlations between blood glucose level and diabetes signs in streptozotocininduced diabetic mice, Global journal of pharmacology, 4, nr. 3,2010, 111-116.
14. Varut, RM, Gîrd, CE, Rotar, LT, Varut, MC CG Pisoschi, Evaluation of Polyphenol and Flavonoid Profiles and the Antioxidant Effect of Carduus acanthoides Hydroalcoholic Extract Compared with Vaccinium Myrtillus in an Animal Model of Diabetes Mellitus, Pharmaceutical Chemistry Journal, 51, nr. 12, 2018, p. 1088-1095.
15. Popescu Renata Maria. Cercetări experimentale asupra unor plante cu potenţial hipoglicemiant, 2014.
16. Popescu F., Popescu, RM, BerbecaruIovan, A, Evaluation of hypoglycemic and hypolipidemic effect of some vegetal extracts, using mice with streptozotocin - experimentally induced diabetes, Current Health Sciences Journal, 40, nr. 2, 2014,52-58
