Prevalence and antimicrobial susceptibility pattern of pseudomonas aeruginosa isolated from clinical samples in Jashore Medical College Hospital.

  • Dr. Seema Saha Assistant professor, Department of Microbiology, Jashore Medical College, Jashore, Bangladesh.
  • Dr. Debasish Dutta Assistant professor, Department of Medicine, Jashore Medical College, Jashore, Bangladesh
  • Dr. Goutam Kumar Ghosh Assistant professor, Department of Medicine, Jashore Medical College, Jashore, Bangladesh
  • Dr Ripa Sinthia Biswas Assistant Professor, Department of Community Medicine, Jashore medical college, Jashore, Bangladesh
  • Dr. Surovi Era Suchi Associate Professor, Department of microbiology, Addin sakina medical college hospital, Jashore, Bangladesh.
Keywords: Pseudomonas aeruginosa, Antibiotic Susceptibility Testing, Antipseudomonal Agents, Tazobactam, Aztreonam, Multi Drug Resistance (MDR)

Abstract

Background: Pseudomonas aeruginosa is one of the leading causes of hospital-acquired infections. Increased resistance in this organism continues to pose a significant threat to patient care because of limited therapeutic options. The main objective of this study was to find out the prevalence and current antimicrobial susceptibility pattern of P. aeruginosa isolates obtained from various clinical samples at a tertiary care hospital.

Methods: The study was conducted in the Bacteriology laboratory of the Department of Microbiology, Jashore Medical College Hospital, Jashore, Bangladesh.  All clinical samples received from various departments from January 2021 to December 2021. The colonies which were grown on culture media were identified by different standard biochemical tests. Antimicrobial susceptibility testing was done using Kirby–the Bauer disc diffusion method and the results were interpreted according to the CLSI guidelines. Quality control of the test was done by standards ATCC strain of P. aeruginosa 27853.

Result: A total of 167 cultured organisms were recorded and analyzed in this study. Among 167 cultured organisms, there were 37 isolates identified as P. aeruginosa. Among the antibiotic sensitivity patterns of P. aeruginosa, we found that the most sensitive drug was colistin 35 (94.59%), followed by levofloxacin 31(83.78%), cefuroxime axetil 29(78.38%), gentamicin 26(70.27%), and each cefperazone+sulbactum & netilmicin has a percentage of 24(64.86%) On the other hand, we found P.aeruginosa showed resistance towards Ofloxacin 24(64.86%), Piperacillin 23(62.16%), Ceftazidime 21(56.76%), Cefoprazone 20(54.05%), Cefipime 20(54.05%), Aztreonam 19(51.35%), Cefaprazone + sulbactum 16(43.24%) and Gentamycin 17(45.00%).

Conclusion: Most of the P. aeruginosa strains were isolated from sputum, urine, respiratory secretions and pus samples and were found to be MDR. Piperacillin-tazobactam was the most sensitive chemotherapeutic agent followed by Colistin and levofloxacin.

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CITATION
DOI: 10.26838/MEDRECH.2022.9.6.638
Published: 2022-11-16
How to Cite
1.
Saha S, Dutta D, Ghosh GK, Biswas RS, Suchi SE. Prevalence and antimicrobial susceptibility pattern of pseudomonas aeruginosa isolated from clinical samples in Jashore Medical College Hospital. Med. res. chronicles [Internet]. 2022Nov.16 [cited 2022Nov.27];9(6):416-24. Available from: https://medrech.com/index.php/medrech/article/view/616
Section
Original Research Article